Scientists are now able to elucidate the microbiome of human diseases, agricultural and other natural, environments. Especially at MR DNA Lab, scientists are dedicated to microbiome research. Their method development has opened doors to research around the world.
This initiative is one component of the MR DNA program and constitutes a major NIH effort to broaden access to rapid assay technologies. This program will fund the development and adaptation of biological assays for use in automated high throughput molecular screening (HTS). It is intended that this initiative promote the development of automated screening projects. High throughput molecular screening (HTS) is the automated, simultaneous testing of thousands of distinct molecular signatures in models of biological mechanisms. Active compounds identified through HTS can provide the starting point in the design of powerful research tools that allow pharmacological probing of basic biological mechanisms, and which can be used to establish the role of a molecular target in a disease process, or, its ability to alter the metabolism or toxicity of a therapeutic. The immense potential of HTS to impact our understanding of biological mechanisms is largely untapped because access to automated screening facilities and large compound libraries is limited in academic, government and non-profit research sectors. Many in vitro biological models are currently used to study biological pathways, the effects of genetic perturbations and to establish a disease association. These can be adapted to high throughput formats for the purpose of screening large collections of biologically active compounds. There are a number of characteristics that make an assay suitable for high throughput approaches. The assay must be robust, reproducible and have a readout that is amenable to automated analysis. In addition, it must be possible to miniaturize the assay, for example; to a 96-well plate (or higher density) format or flow-cytometric approach. Further, the assay protocol should be simple enough for automated handling. A broad range of models share many of these features, including; biochemical assays, cellular models and certain model organisms such as yeast or C. elegans. This initiative will support the development of innovative assays for use in both basic research and in therapeutics development programs, with an emphasis on novelty of assay approach and/or novel targets and mechanisms. (1)
The following list is a basic description of the sequencing services provided by Molecular Research, LP (MR DNA).
Genome sequencing is the process through which we can elucidate the the core information (genes) of the DNA or RNA of the sample, or in the case of whole genome sequencing, the entirety of the information (genes and non-coding sequences).
Metagenomics is a rapidly evolving field through which scientists can elucidate some of the previously hidden insights into the vast array of microscopic life on the planet. Every day, scientists are gaining a better understanding of ecology, evolution, diversity, and functions of the microbial universe thanks to metagenomics, which seeks to help sequence microorganisms in large groups that are often difficult to culture.
Microbial sequencing is the focused sequencing of a single microbe or relatively small group of microbes, in contrast with metagenomics. It can assist in the discovery of genetic variations that support the designing of antimicrobial compounds, vaccines, and even engineered microbes for industrial applications.
Genotyping is the technique through which the variations in an organisms DNA are determined by comparing that organisms DNA to a reference sequence. Genoptyping of an organism also reveals its alleles, the various alternative forms of genes or groups of genes. It plays a very important part in the study of diseases, and in combination with next-generation sequencing technology will help improve treatment methods.
Selective sequencing of coding regions of the genome is an effecient and effective alternative to whole genome sequencing. Exons are the parts of coding regions which control the translation of proteins.
Transcriptome sequencing focuses on the complete array of RNA molecules, which include transfer RNA, messenger RNA, ribosomal RNA, and non-coding RNA. Transcriptome sequencing can help answer questions about gene expression, discovery of novel genes and their functions, classification of diseases, or to help identify targets for drug treatment development.
Amplicon sequencing targets relatively small, specific regions of the genome usually in the hundreds of base pairs. Amplicon sequencing combined with next-generation sequencing allows for thousands of amplicons across many samples to be prepared simultaneously and indexed within hours and often within a single-run.
Bacterial and virus typing is used in the accurate and fast identification and discrimination of strains. Enhancements in bacterial and viral typing can also assist in outbreak identification, surveillance, and in the understanding of transmission, pathogenesis, and evolutionary relationships of the target. Often specific isolates can be sequenced within a day using next-generation sequencing techniques.
De novo Sequencing
De novo is a latin expression meaning “from the beginning”. Hence, de novo sequencing is primarily focused on the sequencing of a novel genome for the first time, or genomes in which large variations are expected, such as genomes with high plasticity. It often requires specialized assembly of sequencing reads, and can be very computationally intensive, though next-generation sequencing has largely reduced the overhead associated with it.
Targeted DNA Sequencing
Targeted DNA sequencing allows the researcher to utilize the specificity of PCR in order to target the genes of their choosing. Targeted DNA sequencing provides the ability to acheive deeper sequencing coverage in order to identify those genes expressed at lower levels that may possibly have been missed by other sequencing methods.
Targeted RNA Sequencing
Targeted RNA sequencing allows the researcher to utilize the specificity of PCR in order to target the genes of their choosing. Targeted RNA sequencing provides the ability to acheive deeper sequencing coverage in order to identify those transcripts expressed at lower levels that may possibly have been missed by other sequencing methods.
Aneuploidy and CNV Analysis
Aneuploidy and Copy-umber variations (CNV) are important factors in the study of genetic disorders, disease, and phylogenetics. Next-generation sequencing has made the study and analysis of aneuploidy and CNV much easier than with previous methods.
Small RNA and miRNA Sequencing
This type of sequencing uses high-throughput methods to sequence miRNA and small RNA, which are important to tissue expression patterns, isoforms, and disease associations.